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Lab Week
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Dates
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Exercises
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Extra visits needed
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1
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Jan. 26-29
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1. Lab orientation (pp. 3-7.)
2. Prepare slides for simple staining of bacteria (pp. 17-19)
after learning aseptic transfer procedures (pp. 10-12.) See PAML 21-26 for
images of stained bacteria.
3. Learn to use and care for the microscope (pp.
21-32.)
4. Study distribution of microorganisms (pp. 35-37 and
handout.)
5. Isolate bacteria by streak plate method (pp. 13-15; PAML
9.)
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2
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Feb. 2-5
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1. Follow up: Examine your streak plate with your TA.
Then isolate single bacterial colonies by repeating the streak plate technique
(pp. 13-15; PAML 9.)
2. Follow up: Observe plates showing distribution of
microorganisms (pp. 35-37; PAML 1-4; and handout.)
3. Learn to do a Gram stain and spore stain (pp. 41-45; PAML
27-28 and 32-33.)
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3
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Feb. 9-12
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1. Follow up: Examine your streak plate with your TA.
If necessary, repeat for practice (pp. 13-15; PAML 9.)
2. Learn basic principles of culture media (pp.
49-54.)
3. Examine bacterial motility (pp. 67-70; PAML 35,
64.)
4. Inoculate media for study of Fungi (pp. 73-79, PERIOD I
procedures.) See PAML 143-150 for images of fungi.
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4
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Feb. 16-19
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1. LAB QUIZ #1 (practical.)
2. Follow up: observe and record culture media results
(pp. 49-54.)
3. Follow up: observe and record motility tube results
(pp. 67-70; PAML 64.)
4. Follow up: continue study of Fungi (pp. 73-79,
PERIOD II procedures; PAML 143-150.)
5. Fermentation (pp. 109-112, PERIOD I procedures; PAML
52-53.)
6. Nitrate reduction (pp. 115-118, PERIOD I procedures; PAML
65-66.)
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5. Fermentation (pp. 109-112, PERIOD II procedures.) Return in
2-4 days to record data. Continue incubation.
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5
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Feb. 23-26
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1. Follow up: Fermentation (pp. 109-112, PERIOD III
procedures; PAML 52-53.)
2. Follow up: Nitrate reduction (pp. 115-118, PERIOD II
procedures; PAML 65-66.)
3. Enzymes (pp. 121-124, PERIOD I procedures.) Also see PAML
pp. 43 (casease), 44 (catalase), 54 (gelatinase), 71-72 (oxidase), and 76-77
(amylase).
4. Anaerobes (pp. 95-99; PERIOD I procedures; PAML
6-8.)
5. Receive and streak mixture of unknown bacteria (pp.
127-128.)
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5. Unknown (pp. 127-128). Return within 1-2 days. Identify two
different isolates from regions where separate colonies can be seen. Streak each
of your two isolates on separate plates and incubate. Mark original plate to
show where you took each isolate; retain this plate to show your TA at next lab.
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6.
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Mar. 1-4
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1. Follow up: Enzymes (pp. 121-124, PERIOD II
procedures.) Also see PAML pp. 43 (casease), 44 (catalase), 54 (gelatinase),
71-72 (oxidase), and 76-77 (amylase).
2. Follow up: Anaerobes (pp. 95-99; PERIOD II
procedures; PAML 6-8.)
3. Follow up: Unknown (pp. 127-128.) Inoculate
diagnostic media to identify your two pure culture isolates. Also use gram stain
and, if appropriate, spore stain. Inoculate each isolate on a separate slant
tube to create a culture you can store if needed – after incubating this
tube for 1-2 days, store it in the refrigerator. If you need to run more tests,
use these slant cultures.
4. Optional: take home a sterile container for water testing
in next lab (after spring break). Water should be collected the same day as your
lab.
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3. Unknown (pp. 127-128). Return as needed to record data. Be
aware of rules about lab access. You may not enter room 207 while TAs from other
lab sections are lecturing, and you may not work in room 207 while other
scheduled labs are in progress – instead, take your materials to another
room as directed.
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Mar. 6-14
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SPRING RECESS
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7
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Mar. 15-18
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1. Follow up: Hand in report on bacterial unknowns (pp.
127-128.)
2. Gram positive cocci: (pp. 153-158, PERIOD I procedures;
PAML 15, 41-42, 44, 46.)
3. Practice pipette technique (pp. 6-8.)
4. Water testing (pp. 163-167, PERIOD I procedures; PAML 12,
52-53, 96.)
5. Food testing (handout.)
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2. Gram positive cocci (pp. 153-158): return in 2-3 days,
follow PERIOD II procedure.
4. Water testing (pp. 163-167): return in 2-3 days, follow
PERIOD II procedure.
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8
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Mar. 22-25
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1. LAB QUIZ #2
2. Follow up: Gram positive cocci: (pp. 153-158, PERIOD
III procedures; PAML 15, 41-42, 44, 46.)
3. Follow up: Water testing (pp. 163-167, PERIOD III
procedures; PAML 12, 52-53, 96.)
4. Follow up: Food testing (handout.)
5. Antibiotics (pp. 131-136, PERIOD I procedures; PAML
93-94.)
6. UV Radiation (pp. 83-85, PERIOD I procedures; PAML 91-92,
and handout.)
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9
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Mar. 29-Apr. 1
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1. Follow up: Antibiotics (pp. 131-136, PERIOD II
procedures; PAML 93-94.)
2. Follow up: UV Radiation (pp. 83-85, PERIOD II
procedures; PAML 91-92, and handout.)
3. Soil plate count (pp. 147-149, PERIOD I
procedures.)
4. Disinfectants and Antiseptics (pp. 103-105, PERIOD I
procedures.)
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1. Antibiotics (pp. 131-136): return in 1-2 days, follow
PERIOD III procedures.
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10
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Apr. 5-8
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1. Follow up: Soil plate count (pp. 147-149, PERIOD II
procedures.)
2. Follow up: Disinfectants and Antiseptics (pp.
103-105, PERIOD II procedures.)
3. Microbial Growth (pp. 57-65, PERIOD I procedures; PAML
83-86.)
4. Isolation of Bacteria (pp. 197-206 and handout.)
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4. Isolation of bacteria (pp. 197-206): return as needed to
record data and transfer cultures.
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11
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Apr. 12-15
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1. Follow up: Isolation of Bacteria (pp. 197-206 and
handout.)
2. Follow up: Microbial Growth (pp. 57-65, PERIOD II
procedures; PAML 83-86.)
3. Bacteriophage Isolation (PAML 87 and handout.)
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1. Isolation of bacteria (pp. 197-206): return as needed to
record data and transfer cultures.
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12
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Apr. 19-22
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1. Follow up: Isolation of Bacteria (pp. 197-206 and
handout.)
2. Follow up: Bacteriophage Isolation (PAML 87 and
handout.)
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1. Isolation of bacteria (pp. 197-206): return as needed to
record data and transfer cultures.
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13
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Apr. 26-29
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1. LAB FINAL (mostly practical)
2. Follow up: hand in report on Isolation of Bacteria
not later than Friday Apr. 30 at noon.
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